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Merck
모든 사진(2)

문서

850500P

Avanti

CHAPS

Avanti Research - A Croda Brand

동의어(들):

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

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About This Item

실험식(Hill 표기법):
C32H58N2O7S
CAS Number:
Molecular Weight:
614.88
UNSPSC 코드:
12161902
NACRES:
NA.25

분석

>99% (TLC)

형태

powder

분자량

614.88 g/mol

포장

pkg of 1 × 1 g (850500P-1g)

제조업체/상표

Avanti Research - A Croda Brand

기술

protein quantification: suitable

배송 상태

dry ice

저장 온도

−20°C

SMILES string

C[C@@]12C(CC[C@@H]2[C@@H](CCC(NCCC[N+](C)(CCCS(=O)([O-])=O)C)=O)C)C3[C@H](O)C[C@]4([H])C[C@H](O)CC[C@]4(C)C3C[C@@H]1O

InChI

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

InChI key

UMCMPZBLKLEWAF-BCTGSCMUSA-N

일반 설명

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry. Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150; aggregation number = 10) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

애플리케이션

CHAPS is suitable to prepare bicelles and to study the processes of liposome solubilization and reconstitution of transport proteins.

포장

20 mL Clear Glass Screw Cap Vial (850500P-1g)

법적 정보

Avanti Research is a trademark of Avanti Polar Lipids, LLC

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Functional reconstitution of the nicotinic acetylcholine receptor by CHAPS dialysis depends on the concentrations of salt, lipid, and protein
Schuerholz T, et al.
Biochemistry, 31(21), 5067-5077 (1992)
Bilayer membrane modulation of membrane type 1 matrix metalloproteinase (MT1-MMP) structure and proteolytic activity
Cerofolini L, et al.
Scientific reports, 6, 29511-29511 (2016)
Liposome solubilization and membrane protein reconstitution using Chaps and Chapso
Cladera J, et al.
European Journal of Biochemistry, 243(3), 798-804 (1997)
Stuart J Cordwell
Methods in molecular biology (Clifton, N.J.), 424, 139-146 (2008-03-29)
Reproducible techniques for the prefractionation of proteins prior to two-dimensional gel electrophoresis (2-DE) are essential for increasing the number of unique proteins that can be identified and assayed following biological experimentation. A simple and robust technique for separating highly soluble
Erik F Kot et al.
Langmuir : the ACS journal of surfaces and colloids, 34(11), 3426-3437 (2018-02-28)
Isotropic phospholipid bicelles are one of the most prospective membrane mimetics for the structural studies of membrane proteins in solution. Recent works provided an almost full set of data regarding the properties of isotropic bicelles; however, one major aspect of

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