220201

CHAPS

Molecular Biology Grade

• 동의어(들): CHAPS, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
• 실험식(Hill 표기법): C₃₂H₅₈N₂O₇S
• CAS Number: 75621-03-3
• Molecular Weight: 614.88
• MDL number: MFCD00012116
• UNSPSC 코드: 12161703
• NACRES: NA.25

속성

• product name: CHAPS, Molecular Biology Grade,
• Grade: Molecular Biology
• Quality Level: 300
• 설명: zwitterionic
• 분석: ≥98% (NMR)
• 형태: crystalline powder
• 분자량: micellar avg mol wt 6150
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated (hygroscopic)
• 응집 번호: 10
• 색상: white
• CMC: 6 - 10 mM; 6 mM (20-25°C)
• mp: 157 °C (315 °F)
• 전이 온도: cloud point >100 °C
• solubility: water: 1.0 M
• 외래 활성: DNase, RNase, and Protease, none detected
• 배송 상태: ambient
• 저장 온도: 15-25°C
• SMILES string: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• InChI key: UMCMPZBLKLEWAF-RFCNGIAKSA-N

설명

일반 설명

Conductivity (1.0 M, 24°C): <100 µmhos.

Non-denaturing zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than the structurally related carboxylic acid anions, and is more effective at breaking protein-protein interactions than either sodium cholate or TRITON^™ X-100. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding to opiates. Also capable of disaggregating P 450 to its monomeric form without denaturation. Has a small micellar molecular weight (6150 Daltons) and a high critical micellar concentration (6-10 mM). Can be removed from either gels or protein solutions by dialysis. Aggregation number: 4-14.

Zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than structurally-related carboxylic acid anions and is much more effective at breaking protein-protein interactions than either sodium cholate or TRITON^™ X-100 Detergent. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding of opiates. Also, capable of disaggregating cytochrome P450 to its monomeric form without denaturation. Has a small micellar molecular weight and a high critical micellar concentration and can be removed from either gels or protein solutions by dialysis across a membrane. Aggregation number: 4-14.

애플리케이션

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

경고

Toxicity: Harmful (C)

재구성

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.

기타 정보

Chen, Y., et al. 1992. Biochemistry 31, 2415.
Ofri, D., et al. 1992. J. Neurochem. 58, 628.
Ransom, R.W., et al. 1992. Biochem. Pharmacol. 43, 1823.
Simonds, W.F., et al. 1992. Proc. Natl. Acad. Sci. USA77, 4623.
Yannariello-Brown, J., and Weigel, P.H. 1992. Biochemistry31, 576.
Hjelmeland, L.M. 1980. Proc. Natl. Acad. Sci. USA77, 6368.
Simonds, W.F., et al. 1980. Proc. Natl. Acad. Sci. USA77, 4623.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302
• 예방조치 성명서: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable