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Merck

1.50657

SeQuant® ZIC®-cHILIC 100Å (3 µm) HPLC Columns

L x I.D. 100 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

동의어(들):

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
41115709
NACRES:
SB.52
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제품 이름

SeQuant® ZIC®-cHILIC 3µm,100Å 100 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC

material

PEEK-lined stainless steel column

product line

SeQuant®

feature

endcapped: no

parameter

550 bar pressure
70 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 2.1 mm

matrix

fully porous particle

matrix active group

ZIC-cHILIC bonding phase

particle size

3 μm

pore size

100 Å pore size

operating pH

1-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

Quality Level

Analysis Note

Pressure: ≤ 40 bar
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.25

General description

SeQuant® ZIC®-cHILIC is designed for excellent HPLC and LC-MS of polar hydrophilic compounds. This zwitterionic stationary phase with phosphorylcholine functional group provides you with complementary selectivity for easier method development for substances that have been difficult to separate by previous types of HPLC columns operated in reversed-phase or HILIC mode.The column dimension of 100 mm length x 2.1 mm ID is the best choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.SeQuant® ZIC®-cHILIC HPLC columns provide complementary selectivity to optimize separations of polar hydrophilic compounds. The columns carry a novel densely bonded, 1:1 charge-balanced phosphorylcholine functional group. As with all true zwitterionic stationary phases, straightforward and reproducible separations are achieved by hydrophilic partitioning combined with weak ionic interactions.The more accessible positive charges of SeQuant® ZIC®-cHILIC columns enable selectivity tuning in chromatography through altered interaction with anionic and cationic molecular moieties. This can be favorable in several situations, for example in the separation of labile metal complexes or highly polar and multiply charged aminoglycosides. Their extraordinary stability and minimized bleed also make SeQuant® ZIC®-cHILIC columns the preferred choice for many LC-MS applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.

프로토콜

HILIC separation is an alternative that permits sensitive MS detection and without the use of ion-pair reagents.

ZIC®-cHILIC is a densely bonded zwitterionic stationary phase with phosphorylcholine functional groups covalently attached to silica.

관련 콘텐츠

저분자는 일반적으로 분자량이 900달톤 미만인 이온 및 화합물입니다. 이러한 화합물은 주로 실리카 입자 또는 다양한 컬럼 화학(변형)을 가진 모놀리식 고정상을 사용하여 HPLC, UHPLC 및 LC-MS로 효과적으로 분리 및 분석할 수 있습니다.

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

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