189730

Streptavidin

• 동의어(들): Streptavidin, Avidin, Streptomyces avidinii
• CAS Number: 9013-20-1
• MDL number: MFCD00082035
• UNSPSC 코드: 41116133
• NACRES: NA.41

속성

• 양식: lyophilized solid
• Quality Level: 100
• 특이 활성도: ≥10 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

A high affinity biotin binding protein (K_d approximately 10^-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.

A high-affinity biotin binding protein.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.

재구성

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.

Reconstitute in H₂O or 1 mM NaHCO₃, pH 9.0 at twice the final concentration and then dilute with an equal volume of 2X buffer.

기타 정보

Eberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
Roth, J., et al. 1992. Histochemistry 98, 229.
Sano, T., et al. 1992. Science 258, 120.
Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
Srivastava, P., et al. 1992. Cytometry 13, 711.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable