추천 제품
재조합
expressed in corn
Quality Level
분석
≥80% protein basis (biuret)
양식
powder
특이 활성도
≥12 units/mg protein (E1%/280)
분자량
glycoprotein 66 kDa
subunit 16 kDa
기술
ELISA: suitable
immunoblotting: suitable
immunoelectrophoresis: suitable
UniProt 수납 번호
저장 온도
2-8°C
유전자 정보
chicken ... AVD(396260)
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일반 설명
Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.
애플리케이션
Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.
단위 정의
One unit will bind 1.0 μg of d-biotin.
제조 메모
Affinity purified
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Sofia H L Frost et al.
Cancer biotherapy & radiopharmaceuticals, 28(2), 108-114 (2012-12-13)
Abstract Purpose: Pretargeted radioimmunotherapy (PRIT) against intraperitoneal (i.p.) ovarian microtumors using avidin-conjugated monoclonal antibody MX35 (avidin-MX35) and (211)At-labeled, biotinylated, succinylated poly-l-lysine ((211)At-B-PLsuc) was compared with conventional radioimmunotherapy (RIT) using (211)At-labeled MX35 in a nude mouse model. Mice were inoculated i.p.
The avidin-biotin complex (ABC) method and other avidin-biotin binding methods.
G L Bratthauer
Methods in molecular biology (Clifton, N.J.), 115, 203-214 (1999-03-31)
Stefan Schillberg et al.
Journal of plant physiology, 258-259, 153359-153359 (2021-01-19)
Recombinant proteins play an important role in many areas of our lives. For example, recombinant enzymes are used in the food and chemical industries and as high-quality proteins for research, diagnostic and therapeutic applications. The production of recombinant proteins is
Commercial Plant-Produced Recombinant Protein Products, 15-25 (2014)
Tom J A Kokhuis et al.
Ultrasound in medicine & biology, 39(3), 490-506 (2013-01-26)
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