235425

Caspase-3 Substrate II, Fluorogenic

Fluorogenic caspase-3 substrate.

• 동의어(들): Caspase-3 Substrate II, Fluorogenic, Ac-DEVD-AMC
• 실험식(Hill 표기법): C₃₀H₃₇N₅O₁₃
• CAS Number: 169332-61-0
• Molecular Weight: 675.64
• MDL number: MFCD00671411
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• Quality Level: 100
• 분석: ≥95% (HPLC)
• 양식: solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; protect from light
• 색상: off-white
• solubility: DMSO: 10 mM
• 형광: λ_ex 365-380 nm; λ_em 430-460 nm
• 배송 상태: ambient
• 저장 온도: −20°C
• SMILES string: N([C@@H](C(C)C)C(=O)N[C@@H](CC(=O)O)C(=O)Nc1cc2[o][c](cc(c2cc1)C)=O)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)CC(=O)O)CCC(=O)O
• InChI: 1S/C30H37N5O13/c1-13(2)26(35-27(44)18(7-8-22(37)38)33-29(46)19(11-23(39)40)31-15(4)36)30(47)34-20(12-24(41)42)28(45)32-16-5-6-17-14(3)9-25(43)48-21(17)10-16/h5-6,9-10,13,18-20,26H,7-8,11-12H2,1-4H3,(H,31,36)(H,32,45)(H,33,46)(H,34,47)(H,35,44)(H,37,38)(H,39,40)(H,41,42)/t18-,19-,20-,26-/m0/s1
• InChI key: ALZSTTDFHZHSCA-RNVDEAKXSA-N

설명

일반 설명

Fluorogenic CPP32/Apopain substrate. Cleavage of this substrate by CPP32/Apopain shows Michaelis-Menten kinetics (K_m = 9.7 ± 1.0 µM). Cleavage can be monitored by excitation at 380 nm and emission at 460 nm.

Fluorogenic caspase-3 substrate. Cleavage of this substrate by caspase-3 shows Michaelis-Menten kinetics (K_m = 9.7 ± 1.0 µM). Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

생화학적/생리학적 작용

Cell permeable: no

Km = 9.7 ± 1.0 µM for cleavage of this substrate by caspase-3

Primary Target
Fluorogenic caspase-3 substrate

Product does not compete with ATP.

Reversible: no

경고

Toxicity: Standard Handling (A)

서열

Ac-Asp-Glu-Val-Asp-AMC

재구성

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

기타 정보

Hermission, M., et al. 2000. Oncogene 19, 2338.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature 376, 37.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable