362185

N-Glycosidase F, Elizabethkingia meningosepticum

• 동의어(들): N-Glycosidase F, Elizabethkingia meningosepticum, Glycopeptidase F, PNGase F
• CAS Number: 83534-39-8
• 효소 위원회 번호: 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD00131222
• UNSPSC 코드: 12352202
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Elizabethkingia meningosepticum)
• Quality Level: 100
• 결합: (N-linked)
• 형태: liquid
• 특이 활성도: ≥20,000 units/mg protein; ≥4500 units/mL
• 제조업체/상표: Calbiochem^®
• 저장 조건: do not freeze
• 외래 활성: Proteases, none detected
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1-3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.

물리적 형태

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

기타 정보

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable