598101

Thioredoxin, Human, Recombinant, E. coli

• 동의어(들): Thioredoxin, Human, Recombinant, E. coli, rhTRX
• CAS Number: 52500-60-4
• MDL number: MFCD00132423
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 분석: ≥90% (SDS-PAGE)
• 형태: lyophilized solid
• 특이 활성도: ≥1.7 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

Recombinant, human thioredoxin expressed in E. coli. A ubiquitous protein with two redox-active half-cysteine residues that function as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman′s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T-cells and human PBL blasts. Occurs as a single polypeptide chain.

Recombinant, human thioredoxin expressed in E. coli. A ubiquitous protein with two redox-active half-cysteine residues. Functions as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman’s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T cells and human PBL.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit (insulin reduction unit) is calculated by measuring the change in absorbance at 650 nm with 330 µM DTT as the reductant at 25°C, pH 6.5.

재구성

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Reconstitute in physiological buffer containing 3 mM EDTA and 1-5 mM DTT.

기타 정보

Noda, N., et al. 1997. Nippon Rinsho 55, 461.
Wollman, E.E., et al. 1997. Biochem. Biophys. Res. Commun. 230, 602.
Masutani, H., et al. 1996. Immunol. Lett. 54, 67.
Saito, I., et al. 1996. Arthritis Rheumatism, 39, 773.
Saito, N., et al. 1995. J. Immunol. 154, 3194.
Buchanon, B.B., et al. 1994. Arch. Biochem. Biophys. 314, 257.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable