추천 제품
Quality Level
사용
kit sufficient for 100 assays (1 mL)
배송 상태
dry ice
저장 온도
−20°C
유전자 정보
human ... TXNRD1(7296) , TXNRD2(10587) , TXNRD3(114112)
일반 설명
The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
애플리케이션
Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).
특징 및 장점
- An easy and simple method
- The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
- The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
- The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells
키트 구성품 전용
제품 번호
설명
- Assay Buffer 5x for Thioredoxin Reductase 30 mL
- Thioredoxin Reductase 10 μg
- Thioredoxin Reductase Inhibitor Solution .05 mL
- 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
- NADPH 25 mg
- Dimethyl Sulfoxide (DMSO) 7.5 mL
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Repr. 2
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
188.6 °F
Flash Point (°C)
87 °C
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시험 성적서(COA)
Lot/Batch Number
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