AB3843

Anti-Ezrin/Radixin/Moesin Antibody

Chemicon^®, from rabbit

• 동의어(들): Anti-EBP50, Anti-NHERF, Anti-NHERF-1, Anti-NHERF1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, monkey, rat, human
• 제조업체/상표: Chemicon^®
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI 수납 번호: NM_004252.2
• UniProt 수납 번호: O14745
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SLC9A3R1(9368)

설명

특이성

Recognizes Ezrin/Radixin/Moesin. These proteins function as linkers between the plasma membrane and the actin cytoskeleton, and are involved in cell adhesion, membrane ruffling, and microvilli formation. This antibody does not cross-react with other related proteins such as merlin or band 4.1.

면역원

KLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin.

애플리케이션

Detect Ezrin/Radixin/Moesin using this Anti-Ezrin/Radixin/Moesin Antibody validated for use in WB, IC, IH(P).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western blotting: 1:1,000. The 75 kDa band is Moesin and the 80kDa band is Ezrin and Radixin. (For best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)

Immunohistochemistry (Paraffin): 1:50. Positive control tissue is breast carcinoma. Cellular localization is membrane and cytoplasm

Immunocytochemistry (IF): 1:100

Optimal working dilutions must be determined by the end user.

물리적 형태

Protein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.

저장 및 안정성

Maintain at -20ºC for 12 months, do not aliquot.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2