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Merck
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Key Documents

AP1064

Sigma-Aldrich

PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb

liquid, Calbiochem®

동의어(들):

Anti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

형태

liquid

포함

≤0.1% sodium azide as preservative

종 반응성

human

제조업체/상표

Calbiochem®

저장 조건

OK to freeze
avoid repeated freeze/thaw cycles

배송 상태

wet ice

저장 온도

−20°C

타겟 번역 후 변형

phosphorylation (pSer300)

일반 설명

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300.
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300 in HEK293 cells.
This PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).

면역원

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α

애플리케이션



Immunoblotting (0.5 μg/ml)
Immunocytochemistry (1 μg/ml)

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

물리적 형태

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.

재구성

Following initial thaw, aliquot and freeze (-20°C).

분석 메모

Negative Control
HEK293 cells treated with dichloroacetate
Positive Control
HEK293 cells

기타 정보

Due to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.
Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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