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Merck
모든 사진(1)

문서

AP1063

Sigma-Aldrich

PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb

liquid, Calbiochem®

동의어(들):

Anti-Pyruvate Dehydogenase pSer²³² Rabbit pAb

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

형태

liquid

포함

≤0.1% sodium azide as preservative

종 반응성

mouse, human, rat

제조업체/상표

Calbiochem®

저장 조건

OK to freeze
avoid repeated freeze/thaw cycles

배송 상태

wet ice

저장 온도

−20°C

타겟 번역 후 변형

phosphorylation (pSer232)

일반 설명

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232.
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232 in HEK293 cells.
This PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb is validated for use in Immunoblotting, IF, IP for the detection of PDH-E1α (pSer²³²).

면역원

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α

애플리케이션



Immunoblotting (0.25 g/ml)
Immunofluorescence (Please see comments)
Immunoprecipitation (1 g/ml)

경고

Toxicity: Standard Handling (A)

물리적 형태

In PBS.

재구성

Following initial thaw, aliquot and freeze (-20°C).

분석 메모

Positive Control
HEK293 cells

기타 정보

Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
This antibody has been known to work with immunofluorescence. Antibody should be titrated for optimal results in individual systems.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Lærke Bertholdt et al.
Journal of applied physiology (Bethesda, Md. : 1985), 124(3), 729-740 (2017-12-02)
Recruitment of fatty acids from adipose tissue is increased during fasting. However, the molecular mechanisms behind fasting-induced metabolic regulation in human adipose tissue and the potential impact of training state in this are unknown. Therefore the aim of the present
Shelby L Oke et al.
Reproduction (Cambridge, England), 159(1), 27-39 (2019-11-07)
Epidemiological data suggest an inverse relationship between birth weight and long-term metabolic deficits, which is exacerbated by postnatal catch-up growth. We have previously demonstrated that rat offspring subject to maternal protein restriction (MPR) followed by catch-up growth exhibit impaired hepatic
Arpit Sharma et al.
eLife, 8 (2019-07-16)
Metabolic cycles are a fundamental element of cellular and organismal function. Among the most critical in higher organisms is the Cori Cycle, the systemic cycling between lactate and glucose. Here, skeletal muscle-specific Mitochondrial Pyruvate Carrier (MPC) deletion in mice diverted
Rosa Ferriero et al.
Science translational medicine, 5(175), 175ra31-175ra31 (2013-03-08)
Lactic acidosis is a buildup of lactic acid in the blood and tissues, which can be due to several inborn errors of metabolism as well as nongenetic conditions. Deficiency of pyruvate dehydrogenase complex (PDHC) is the most common genetic disorder
Charandeep Singh et al.
Nature communications, 11(1), 1277-1277 (2020-03-11)
Although supplemental oxygen is required to promote survival of severely premature infants, hyperoxia is simultaneously harmful to premature developing tissues such as in the retina. Here we report the effect of hyperoxia on central carbon metabolism in primary mouse Müller

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