GF003

Fibroblast Growth Factor basic, human

>95% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture

• 동의어(들): FGF-2, Heparin-Binding Growth Factor 2, bFGF, beta-FGF
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

속성

• 제품명: Fibroblast Growth Factor basic Protein, Human recombinant,
• 생물학적 소스: human
• Quality Level: 100
• 분석: >95% (SDS-PAGE)
• 제조업체/상표: Chemicon^®
• 기술: cell culture | stem cell: suitable
• 불순물: <0.1 EU/μg Endotoxin level (of FGF-b)
• 입력: sample type neural stem cell(s); sample type mesenchymal stem cell(s); sample type epithelial cells
• NCBI 수납 번호: NM_002006.4
• UniProt 수납 번호: P09038

설명

일반 설명

Fibroblast Growth Factor-basic (FGF-b) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-b also exerts a potent angiogenic activity in vivo. Human FGF-b is a 17.2 kDa protein containing 155 amino acid residues.

Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. Xu, et al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions. GF003-AF was developed without animal-based ingredients and can be used for the culture of hES cells in a feeder-free, animal-free culture system.

expressed in E. coli

애플리케이션

For most in vitro applications, bFGF exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Endothelial, mesenchymal cells. Human ES cells require concentrations in the range of 4 to 100 ng/mL, depending on the method of culture.

Optimal working dilutions must be determined by end user.

The Fibroblast Growth Factor-basic (FGF-b) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.

결합

FA009-1

물리적 형태

Lyophilized from a 0.7 mg/mL solution in 5mM Tris, pH 7.6 with 150 mM NaCl.

저장 및 안정성

Maintain the lyophilized material at -20°C until expiration date as stated on the label.


General applications:

After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted b

분석 메모

Chemicon′s Human FGF-b is fully biologically active when compared to standards. The ED₅₀ was determined by the dose-dependent stimulation of thymidine uptake by NIH3T3 cells expressing FGF receptors.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable