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Merck
모든 사진(1)

주요 문서

GF144

Sigma-Aldrich

Epidermal Growth Factor Protein, Human recombinant

>98% (SDS-PAGE), recombinant, expressed in E. coli

동의어(들):

EGF

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About This Item

UNSPSC 코드:
12352202
eCl@ss:
32160405
NACRES:
NA.77

product name

Epidermal Growth Factor Protein, Human recombinant, The Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.

Quality Level

분석

>98% (SDS-PAGE)

특이 활성도

≥1 x 10^7 U/mg

제조업체/상표

Chemicon®

불순물

<0.1 ng/μg Endotoxin (of EGF; 1EU/μg)

입력

sample type neural stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

일반 설명

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.
Product Source: E.coli

애플리케이션

For most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.

결합

Replaces: GF001

물리적 형태

Lyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.

저장 및 안정성

The lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.

분석 메모

Specific Activity: Human EGF is fully biologically active when compared to standards.The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.1 ng/mL

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


시험 성적서(COA)

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문서 라이브러리 방문

Lenka Munoz et al.
Cancer biology & therapy, 17(4), 355-363 (2016-01-23)
Inflammation is an important factor promoting the progression of glioblastoma. In the present study we examined the contribution of Ras signaling and TNFα/IL-1β cytokines to the development of the glioblastoma inflammatory microenvironment. Enhanced activation of Ras through de-regulated activation of
Seonhee Kim et al.
Frontiers in microbiology, 12, 730892-730892 (2022-01-01)
Rabies is a zoonotic disease caused by rabies virus (RABV). As rabies advances, patients develop a variety of severe neurological symptoms that inevitably lead to coma and death. Unlike other neurotropic viruses that can induce symptoms of a similar range
Hui-Fang Guo et al.
Antioxidants (Basel, Switzerland), 9(2) (2020-02-08)
Background: An experimental study was undertaken to determine the efficacy of the epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream in the wound-healing process on skin with deep partial-thickness burn in rats. Methods: A total of 180 Sprague-Dawley rats
Weili Ma et al.
Nanomaterials (Basel, Switzerland), 9(3) (2019-03-21)
Magnetic iron oxide (Magnetite, Fe₃O₄) nanoparticles are widely utilized in magnetic resonance imaging (MRI) and drug delivery applications due to their superparamagnetism. Surface coatings are often employed to change the properties of the magnetite nanoparticles or to modulate their biological
GUSBP11 Inhibited The Progression of Triple Negative Breast Cancer via Targeting The miR-579-3p/SPNS2 Axis.
Wu, et al.
Cell Journal, 24, 230-238 (2022)

프로토콜

Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.

관련 콘텐츠

This article shows that a PES membrane containing Stericup Quick Release filter provides an alternative for the pluripotent expansion of embryonic stem cells.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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