MABN504

Anti-VDAC1 Antibody, clone N152B/23

clone N152B/23, from mouse

• 동의어(들): Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: N152B/23, monoclonal
• 종 반응성: rat, mouse, human
• 포장: antibody small pack of 25 μg
• 기술: immunohistochemistry: suitable; western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_003365
• UniProt 수납 번호: P21796
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... VDAC1(7416)

설명

일반 설명

Voltage-dependent anion-selective channel protein 1 (VDAC1, VDAC), also known as outer mitochondrial membrane protein porin 1, is the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC1 forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation and apoptosis. VDAC1 may participate in the formation of the permeability transition pore complex (PTPC), which is responsible for the release of mitochondrial products that triggers apoptosis.

특이성

This antibody does not cross-react with VDAC2 or VDAC3 (Prof. J. Trimmer, University of California, Davis).

면역원

Recombinant protein corresponding to human VDAC1.

애플리케이션

Detect VDAC using this mouse monoclonal antibody, Anti-VDAC1 Antibody, clone N152B/23 validated for use in western blotting & IHC.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: A representative lot states VDAC1 does not cross-react with VDAC2 or VDAC3 (based on KO validation results) representative lot deteted VDAC1 in wild type mouse liver tissue, and demonstrated a loss of signal in VDAC1 knock out mouse hippocampal tissue using immunobloting analysis (Prof. J. Trimmer, University of California, Davis).
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected VDAC1 in human cardiac myocytes tissue.

품질

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected VDAC1 in 10 µg of mouse brain tissue lysate.

표적 설명

~ 35 kDa observed

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Mouse brain tissue lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable