추천 제품
생물학적 소스
rat
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
78, monoclonal
종 반응성
mouse, human, rat
기술
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... TREM2(54209)
관련 카테고리
일반 설명
TREM-2, also known as Triggering receptor expressed on myeloid cells 2 or Triggering receptor expressed on monocytes 2, and encoded by the gene Trem2/Trem2a/Trem2b/Trem2c, is a protein that forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. TREM-2 may also have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM-2 is localized to the cell membrane and is a single pass membrane protein. TREM-2 is expressed in immune cells such as microglia and monocytes but the expression varies depending upon the exact tissue and location within the tissue. Defects in TREM-2 may be associated in humans with frontotemporal dementia, Nasu-Hakola disease, and recently an increase risk of Alzheimer′s disease.
특이성
This antibody does not cross react with mouse TREM-1 or TREM-3 (Prof. William Seaman, University of California, San Francisco.).
면역원
Recombinant protein corresponding to mouse TREM-2.
애플리케이션
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected TREM-2 in TRACP+ osteoclasts (Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected TREM-2 in RAW264.7 cells (Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated TREM-2 from mouse macrophage lysates (Peng, Q., et al. (2010). Sci Signal. 3(122):ra38.).
Inhibition Analysis: : Pre-treatment of BV2 cells or C57BL/6 Osteoclasts with a representative lot of this antibody significantly reduces cellular activation in response to both untreated and apoptotic Neuro2A cells (Hsieh, C. L., et al. (2009). J Neurochem. (109):4:1144-1156.; Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected TREM-2 in RAW264.7 cells (Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated TREM-2 from mouse macrophage lysates (Peng, Q., et al. (2010). Sci Signal. 3(122):ra38.).
Inhibition Analysis: : Pre-treatment of BV2 cells or C57BL/6 Osteoclasts with a representative lot of this antibody significantly reduces cellular activation in response to both untreated and apoptotic Neuro2A cells (Hsieh, C. L., et al. (2009). J Neurochem. (109):4:1144-1156.; Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-TREM-2 Antibody, clone 78 is validated for use in western blotting, ICC, flow cytometry, inhibition & IP for the detection of TREM-2.
품질
Evaluated by Western Blotting in human spleen tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TREM-2 in 10 µg of human spleen tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TREM-2 in 10 µg of human spleen tissue lysate.
표적 설명
~25 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
물리적 형태
Format: Purified
Protein G Purified
Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Peng Zhao et al.
Molecular neurodegeneration, 17(1), 44-44 (2022-06-19)
Microglia plays crucial roles in Alzheimer's disease (AD) development. Triggering receptor expressed on myeloid cells 2 (TREM2) in association with DAP12 mediates signaling affecting microglia function. Here we study the negative regulation of TREM2 functions by leukocyte immunoglobulin-like receptor subfamily
Yingjun Zhao et al.
Neuron, 97(5), 1023-1031 (2018-03-09)
Mutations in triggering receptor expressed on myeloid cells 2 (TREM2) have been linked to increased Alzheimer's disease (AD) risk. Neurobiological functions of TREM2 and its pathophysiological ligands remain elusive. Here we found that TREM2 directly binds to β-amyloid (Aβ) oligomers
Shih-Wei Chen et al.
Methods in molecular biology (Clifton, N.J.), 2683, 53-68 (2023-06-10)
The generation of a homogeneous population of microglia from human induced pluripotent stem cells (hiPSCs) is crucial to modeling neurological disorders, as well as the carrying out of drug screening and toxicity testing. Here, we provide a stepwise protocol for
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