10280879001

5-Bromo-2′-deoxyuridine

>97%, crystalline solid, pkg of 1 g

• 동의어(들): 5-Bromo-2′-deoxyuridine, brdu, 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
• 실험식(Hill 표기법): C₉H₁₁BrN₂O₅
• CAS Number: 59-14-3
• Molecular Weight: 307.10
• Beilstein: 30395
• MDL number: MFCD00006529
• UNSPSC 코드: 12352204
• PubChem Substance ID: 329749004

속성

• Quality Level: 100
• 분석: >97%
• 형태: crystalline solid
• 분자량: 307.1
• 포장: pkg of 1 g
• 제조업체/상표: Roche
• mp: 191-194 °C (dec.) (lit.)
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• SMILES string: OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
• InChI key: WOVKYSAHUYNSMH-RRKCRQDMSA-N

설명

일반 설명

It is conventionally used as a marker for S-phase synthesis of DNA.

애플리케이션

5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.

Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.

생화학적/생리학적 작용

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

품질

Purity: 97% (from N), chromatographically homogeneous

제조 메모

Working concentration: According to literature for cell culture, a final concentration of 10 μmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.

Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H340,H361fd
• 예방조치 성명서: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 1B - Repr. 2
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable