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Merck
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문서

10843555001

Roche

Hygromycin B

from Streptomyces hygroscopicus

동의어(들):

Hygromycin B from Streptomyces hygroscopicus

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모든 사진(2)

About This Item

실험식(Hill 표기법):
C20H37N3O13
CAS Number:
31282-04-9
Molecular Weight:
527.52
MDL number:
MFCD06795479
UNSPSC 코드:
12352200
PubChem Substance ID:
329749191

생물학적 소스

Streptomyces hygroscopicus

무균

non-sterile; 0.2 μm filtered

분석

80% (HPLC)

형태

buffered aqueous solution

구성

Hygromycin B, >80% HPLC

포장

pkg of 20 mL (1 g)

제조업체/상표

Roche

농도

50 mg/mL

기술

transfection: suitable

SMILES string

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

  • Formula: C20H37N3O13
  • LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
  • Molecular weight: Mr = 527.5
  • Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.

애플리케이션

Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).

생화학적/생리학적 작용

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

품질

Purity: >80% (HPLC)

단위 정의

Unit Assay: Unit/mg
We do not have this information.

물리적 형태

Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane

제조 메모

Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.

저장 및 안정성

Store at 2 to 8 °C. (solution)

기타 정보

For life science research only. Not for use in diagnostic procedures.
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.

픽토그램

CorrosionSkull and crossbonesHealth hazard
GHS05,GHS06,GHS08

신호어

Danger

유해 및 위험 성명서

H301,H312,H317,H318,H330,H334

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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