추천 제품
사용
sufficient for ≤2,000 tests
Quality Level
포장
pkg of 1 kit
제조업체/상표
Roche
기술
protein quantification: suitable
λmax
490 nm
응용 분야
cell analysis
detection
검출 방법
colorimetric
저장 온도
−20°C
일반 설명
A nonradioactive alternative to the [51Cr] and [3H]-thymidine release assay.
Colorimetric assay for the quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant.
Colorimetric assay for the quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant.
애플리케이션
The Cytotoxicity Detection Kit (LDH) is a fast and simple method to quantify cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well plate format. Thus, the kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example:
- Detection and quantification of cell-mediated cytotoxicity
- Determination of mediator-induced cytolysis
- Determination of the cytotoxic potential of compounds in environmental and medical research, and in the food, cosmetic, and pharmaceutical industries
- Determination of cell death in bioreactors
- Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth
포장
1 kit containing 2 components.
제조 메모
Working solution: Vial 1 Catalyst:
Reconstitute the lyophilisate in 1 ml double dist. water for 10 minutes and mix thoroughly.
Vial 2 Dye solution:
Ready-to-use solution.
Reaction mixture
For 100 tests: Shortly before use, mix 250 μl of vial 1 with 11.25 ml of vial 2.
For 400 tests: Shortly before use, add the total volume of catalyst (1 ml) to the total volume
of dye solution (45 ml) and mix well.
Note: The Reaction mixture should not be stored; prepare immediately before use.
Storage conditions (working solution): 2 to 8 °C
The lyophilizate is stable at 2 to 8 °C.
The reconstituted catalyst solution is stable for four weeks when stored at 2 to 8 °C.
Once thawed, the dye solution is stable for several weeks when stored at 2 to 8 °C.
Reconstitute the lyophilisate in 1 ml double dist. water for 10 minutes and mix thoroughly.
Vial 2 Dye solution:
Ready-to-use solution.
Reaction mixture
For 100 tests: Shortly before use, mix 250 μl of vial 1 with 11.25 ml of vial 2.
For 400 tests: Shortly before use, add the total volume of catalyst (1 ml) to the total volume
of dye solution (45 ml) and mix well.
Note: The Reaction mixture should not be stored; prepare immediately before use.
Storage conditions (working solution): 2 to 8 °C
The lyophilizate is stable at 2 to 8 °C.
The reconstituted catalyst solution is stable for four weeks when stored at 2 to 8 °C.
Once thawed, the dye solution is stable for several weeks when stored at 2 to 8 °C.
키트 구성품 전용
제품 번호
설명
- Catalyst (Diaphorase/NAD+ mixture)
- Dye Solution (INT and sodium lactate)
관련 제품
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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문서
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
관련 콘텐츠
Cytotoxicity Detection Kit (LDH) Protocol & Troubleshooting
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