TOX7

In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based

• 동의어(들): LDH cytotoxicity assay
• UNSPSC 코드: 12352207
• NACRES: NA.84

속성

• 형태: liquid
• Quality Level: 200
• 사용: kit sufficient for 500 tests
• 포장: pkg of 1 kit
• 저장 조건: dry at room temperature
• 기술: protein quantification: suitable
• λmax: 490 nm
• 응용 분야: cell analysis; detection
• 검출 방법: colorimetric
• 저장 온도: −20°C

설명

일반 설명

Tox7 is a member of Sigma′s CytoToxicity Kit series. It is based on the measurement of lactate dehydrogenase (LDH) release from cells as a measure of cell membrane damage and cytotoxicity. This technique has been utilized as an alternative to 51Cr release for cell mediated cytotoxicity assays, as well as conventional cytotoxicity resulting from interaction of a test material with the cell.

애플리케이션

In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based has been used:

• to detect lactate dehydrogenase release in human umbilical vein endothelial cells (HUVECs) and human cardiac myocytes (HCMs) for lactate dehydrogenase (LDH) assay
• to determine the activity of lactate dehydrogenase leakage of MDA-MB-231 cells
• to examine the activity of LDH in RAW 264.7 cells , in LDH cytotoxicity assay in human cell lines
• for spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 490 nm.

생화학적/생리학적 작용

The lactate dehydrogenase assay is a means of measuring either the number of cells via total cytoplasmic lactate dehydrogenase (LDH) or membrane integrity as a function of the amount of cytoplasmic LDH released into the medium. This method is simple, accurate and yields reproducible results. The assay is based on the reduction of NAD by LDH. The resulting reduced NAD (NADH) is utilized in the stoichiometric conversion of a tetrazolium dye to a soluble, colored formazan derivative.
.

안전성 정보

• 유해 및 위험 성명서: H412
• 예방조치 성명서: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 10 - Combustible liquids