추천 제품
양식
solution
Quality Level
사용
sufficient for ≤2,500 tests (11644807001)
sufficient for ≤800 tests (05015944001)
포장
bottle of 25 mL (11644807001)
bottle of 8 mL (05015944001)
제조업체/상표
Roche
저장 조건
protect from light
기술
protein quantification: suitable
최적 pH
8.0(for physiological conditions our product is buffered pH 7.3)
λmax
440-480 nm
응용 분야
cell analysis
detection
검출 방법
colorimetric
저장 온도
−20°C
일반 설명
Ready-to-use solution, containing WST-1 and an electron coupling reagent.
애플리케이션
- Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds
- Assessment of growth inhibitory antibodies and physiological mediators
특징 및 장점
- Convenient: Benefit from a ready-to-use reagent.
- Safe and Easy: Eliminate radioactive isotopes, washing steps, and additional reagents.
- Accurate: The absorbance obtained strongly correlates to the cell number.
- Sensitive: Detect low cell numbers.
- Fast: Process a large number of samples using a multi-well ELISA reader.
원리
Cells grown in a 96-well tissue culture plate are incubated with the WST-1 reagent for 0.5 - 4 hours. After this incubation period, the formazan dye formed is quantitated with a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells.
제조 메모
Using the 100 μl/well cell culture volume, one vial will be sufficient to perform 2500 tests (25 microplates).
Note: If the cells are cultured in 200 μl/well, add 20 μl/well Cell Proliferation Reagent WST-1.
Storage conditions (working solution): 2 to 8 °C
Note: When precipitates or turbidity are observed upon thawing, warm up the solution to 37 °C for 2 to 10 minutes and agitate to dissolve the precipitates.Centrifugation is not recommended because the working concentration would decrease. After being dissolved, the WST-1 reagent can be used without any limitations. Please store as follows:
Once thawed, store at 2 to 8 °C, protected from light, for up to four weeks. However please note that the solution may become viscous. If so, warm up the solution to 37 °C for 2 to 10 minutes as described above.
Long storage
For long storage, aliquots of WST-1 can be stored in plastic tubes at -15 to -25 °C until the expiry date.
기타 정보
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
No data available
Flash Point (°C)
No data available
이미 열람한 고객
문서
암, 신경과학, 줄기 세포 연구 응용분야를 위한 세포 증식(BrdU, MTT, WST1), 세포 생존성, 세포 독성 실험용 세포 기반 분석법.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
프로토콜
WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.