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Merck
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TOX1

Sigma-Aldrich

In Vitro Toxicology Assay Kit, MTT based

동의어(들):

mitochondrial activity assay

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.84

사용

1 kit sufficient for 1,000 tests

Quality Level

200

포장

pkg of 1 kit

저장 조건

dry at room temperature

λmax

570 nm

응용 분야

cell analysis
detection

검출 방법

colorimetric

저장 온도

2-8°C

일반 설명

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.

애플리케이션

In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.

생화학적/생리학적 작용

Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

신호어

Danger

유해 및 위험 성명서

H225,H314,H335,H336,H341,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3

표적 기관

Central nervous system, Respiratory system

Storage Class Code

3 - Flammable liquids

Flash Point (°F)

53.6 °F

Flash Point (°C)

12 °C

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
SLCR1314
SLCR1315
SLCL3638
SLCJ1228
SLCG9357

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Luminescent lanthanide downconversion nanoparticles (DCNPs) provide a combination of high luminescence intensity, sharp emission peaks with narrow bandwidth and a large Stokes' shift, leading to high-performance biomedical applications mainly for imaging. The purpose of this study is to present a
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Drug sensitivity assays were performed using a variation of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] assay on V79, CHO-AuxB1, CHRC5, NCI-H460, and NCI-H249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with
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암, 신경과학, 줄기 세포 연구 응용분야를 위한 세포 증식(BrdU, MTT, WST1), 세포 생존성, 세포 독성 실험용 세포 기반 분석법.

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자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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