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Merck
모든 사진(1)

주요 문서

TOX6

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Sulforhodamine B based

동의어(들):

biomass viability assay, sulforhodamine B, total biomass assay

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.84

양식

liquid

Quality Level

사용

 kit sufficient for 1,000 tests

포장

pkg of 1 kit

저장 조건

dry at room temperature

λmax

565 nm

응용 분야

cell analysis
detection

검출 방법

colorimetric

저장 온도

room temp

일반 설명

The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.

애플리케이션

In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.

생화학적/생리학적 작용

Dye binds to cellular protein and is then solubilized in base.

픽토그램

FlameCorrosionEnvironment

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

Storage Class Code

3 - Flammable liquids


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문서 라이브러리 방문

P Skehan et al.
Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
Santos F, eta l.
Endocrine-related cancer, 21(1), 1-40 (2014)
Marike Gabrielson et al.
PloS one, 9(9), e107109-e107109 (2014-09-23)
Epithelial ovarian carcinoma (EOC), the major cause of gynaecological cancer death, is a heterogeneous disease classified into five subtypes. Each subtype has distinct clinical characteristics and is associated with different genetic risk factors and molecular events, but all are treated
Evaluation of colorimetric protein and biomass stains for assaying drug effects upon human tumor cell lines.
Skehan, P., et al.
Proceedings of the American Association For Cancer Research, 30, 2436-2436 (1989)
Eduardo C A Silva et al.
Oncotarget, 9(29), 20386-20398 (2018-05-15)
Metabolic reprogramming is one of the hallmarks of cancer. The hyperglycolytic phenotype is often associated with the overexpression of metabolism-associated proteins, such as monocarboxylate transporters (MCTs). MCTs are little explored in germ cell tumors (GCTs), thus, the opportunity to understand

문서

암, 신경과학, 줄기 세포 연구 응용분야를 위한 세포 증식(BrdU, MTT, WST1), 세포 생존성, 세포 독성 실험용 세포 기반 분석법.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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