94448

Gram′s crystal violet solution

for microscopy

• 동의어(들): Basic violet 3; gentian violet; hexamethyl pararosaniline; methyl violet 10B
• UNSPSC 코드: 12171500
• NACRES: MA.02

속성

• Grade: for microscopy
• Quality Level: 200
• 제품 라인: BioChemika
• 형태: liquid
• 유통기한: limited shelf life, expiry date on the label
• 기술: microbe id | staining: suitable
• 색상: purple to very dark purple
• density: 0.981 g/mL at 20 °C
• εmax: 33.8 at 585-595 nm in water
• 적합성: suitable for microscopy
• 항생제 활성 스펙트럼: Gram-negative bacteria; Gram-positive bacteria
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp

설명

일반 설명

Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.

애플리케이션

• Crystal violet is routinely used for Gram staining and its variants, staining amyloid, bacterial components, and vascular plant tissues.
• It is used in polychrome staining of epoxy resin sections, viability staining of cultured neurons, and confocal optical sectioning to analyze meiotic structures.
• It is also employed in the acridine orange-crystal violet staining of intracellular bacteria, microsporidian spores, and cytological smears.

생화학적/생리학적 작용

The antimicrobial effects of crystal violet are caused by free radical generation and the formation of unionized dye-bacteria complexes. It also inhibits bacterial protein synthesis and cell wall formation and is especially effective against Gram-positive bacteria.

원리

Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms complexes with Gram’s iodine that are entrapped and retain the purple color even after decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner and does not retain crystal violet.

안전성 정보

• 픽토그램: GHS02,GHS08,GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H226,H319,H351,H412
• 예방조치 성명서: P202 - P210 - P233 - P273 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 3
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point (°F): 93.2 °F
• Flash Point (°C): 34 °C
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter