A9056

Bovine Serum Albumin

heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)

• 동의어(들): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC 코드: 12352202
• NACRES: NA.27

속성

• 생물학적 소스: bovine
• Quality Level: 200
• 분석: ≥96% (agarose gel electrophoresis)
• 형태: lyophilized powder
• 분자량: ~66 kDa
• 정제법: heat shock fractionation
• 포장: poly bottle of
• 기원: USA origin
• 기술: cell culture | mammalian: suitable
• 불순물: ≤5.0% Loss on drying
• 손실: ≤5%
• pH: 7
• solubility: water: soluble (40 mg/ml)
• UniProt 수납 번호: P02769
• 외래 활성: BT virus, none detected; Microbiological test (pass); VSV virus, none detected; growth of Borrelia burgdorferi in BSK II medium (Suitable for)
• 저장 온도: 2-8°C
• 유전자 정보: bovine ... ALB(280717)

설명

일반 설명

Albumin is the most abundant plasma protein in humans. High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Serum albumin is a reliable prognostic indicator for liver disease. Oxidative damage of albumin is associated with advanced liver disease.

애플리케이션

Bovine Serum Albumin has been used as a supplement in Dulbecco′s modified eagle medium (DMEM). It has also been used in immunostaining of cell arrays.

생화학적/생리학적 작용

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

적합성

Suitable for growth of Borrelia burgdorferi in BSK II medium

제조 메모

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)