D0632

DL-Dithiothreitol

≥98% (HPLC), ≥99.0% (titration)

• 동의어(들): (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
• Linear Formula: HSCH₂CH(OH)CH(OH)CH₂SH
• CAS Number: 3483-12-3
• Molecular Weight: 154.25
• Beilstein: 1719757
• EC Number: 222-468-7
• MDL number: MFCD00004877
• UNSPSC 코드: 12352201
• PubChem Substance ID: 24893251
• NACRES: NA.21

속성

• Quality Level: 200
• 분석: ≥98% (HPLC); ≥99.0% (titration)
• 양식: powder
• 반응 적합성: reagent type: reductant
• 색상: white
• mp: 41-44 °C (lit.)
• solubility: H₂O: soluble 50 mg/mL, clear, colorless to very faintly yellow
• 응용 분야: general analytical
• 저장 온도: 2-8°C
• SMILES string: O[C@H](CS)[C@H](O)CS

설명

일반 설명

Dithiothreitol (DTT) is a disulfide (SH) reducing agent found in proteins and in the amino acid cysteine. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange. It is almost 7-fold stronger reducing agent than βME, hence efficient in lower concentrations.

애플리케이션

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

DTT has been used:

• as one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk.[1]
• as a component of medium for the demembranation and reactivation of spermatozoa.[2]
• to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.[3]
• as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.[4]
• in proteomics analysis as in-solution protein digestion for mass spectrometry[5]
• as a buffer component for protein quantification, to prepare wash buffer, lysis buffer, sample buffer, and protein elution buffer[6][4]

생화학적/생리학적 작용

Dithiothreitol (DTT) is extensively applied in chemical peptide synthesis and biochemical preparations of thiol proteins. It is also used in protein chemistry studies, such as protein folding and enzyme activity. It also acts as an enzyme stabilizer to stop DNA modification. DTT specifically mediates the thiol-disulfide interchange reaction to entirely reduce the intra- or inter-molecular disulfide bonds in biomolecules. This reaction results in the formation of thiols and the cyclic disulfide of DTT.[7]

특징 및 장점

• High-quality DTT (HPLC≥98%), (titration≥99.0%)
• Suitable for electrophoresis, proteomics analysis

기타 정보

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안전성 정보

• 픽토그램: GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H318
• 예방조치 성명서: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves