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D5545

Sigma-Aldrich

DL-Dithiothreitol

BioXtra, ≥99.0% (titration)

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Synonym(s):
threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
Linear Formula:
HSCH2CH(OH)CH(OH)CH2SH
CAS Number:
Molecular Weight:
154.25
Beilstein/REAXYS Number:
1719757
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

product line

BioXtra

Quality Level

assay

≥99.0% (titration)

form

powder

reaction suitability

reagent type: reductant

impurities

Insoluble matter, passes filter test
≤0.3% 4,5-dihydroxy-1,2-dithiane

pH

4.0-6.0 (20-25 °C, 0.1 m in H2O)

mp

41-44 °C (lit.)

solubility

H2O: 0.1 M, clear, colorless

anion traces

sulfate (SO42-): ≤0.005%

cation traces

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.1%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorption

≤0.100 at 280 in H2O at 0.1 M
≤0.400 at 260 in H2O at 0.1 M

storage temp.

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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1 of 4

This Item
D9163D977943816
vibrant-m

D5545

DL-Dithiothreitol

Premium Grade
vibrant-m

D9163

DL-Dithiothreitol

-
vibrant-m

D9779

DL-Dithiothreitol

-
vibrant-m

43816

DL-Dithiothreitol solution

Premium Grade
product line

BioXtra

product line

-

product line

-

product line

BioUltra

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

mp

41-44 °C (lit.)

mp

40-44 °C, 41-44 °C (lit.)

mp

41-44 °C (lit.)

mp

-

anion traces

sulfate (SO42-): ≤0.005%

anion traces

-

anion traces

-

anion traces

-

solubility

H2O: 0.1 M, clear, colorless

solubility

H2O: 50 mg/mL, clear, colorless to very faintly yellow

solubility

H2O: 50 mg/mL

solubility

-

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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