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Merck
모든 사진(2)

주요 문서

E7889

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt solution

for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

동의어(들):

(Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2

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About This Item

Linear Formula:
[-CH2N(CH2CO2Na)CH2CO2H]2
CAS Number:
Molecular Weight:
336.21
Beilstein:
3822669
MDL number:
UNSPSC 코드:
12352107
PubChem Substance ID:
NACRES:
NA.31

Grade

for molecular biology

Quality Level

무균

0.2 μm filtered

양식

liquid

반응 적합성

reagent type: chelator

농도

0.5 M in H2O

pH

7.5-8.5

적합성

suitable for molecular biology

응용 분야

microbiology

외래 활성

DNase, RNase, NICKase and protease, none detected

SMILES string

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

InChI key

ZGTMUACCHSMWAC-UHFFFAOYSA-L

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일반 설명

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.
Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.

애플리케이션

Ethylenediaminetetraacetic acid disodium salt solution has been used:
  • in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
  • with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
  • in pretreatment for detaching epithelial cells grown to 80% confluency
  • to prepare stock solutions for lysis buffer
  • in the preparation of urea lysis buffer

특징 및 장점

molecular biology grade

제조 메모

Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

기타 정보

For additional information on our range of Biochemicals, please complete this form.

픽토그램

Health hazard

신호어

Warning

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

STOT RE 2 Inhalation

표적 기관

Respiratory Tract

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

프로토콜

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