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Merck
모든 사진(1)

주요 문서

P7170

Sigma-Aldrich

Ponceau S solution

BioReagent, 0.1 % (w/v) in 5% acetic acid

동의어(들):

Ponceau S

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About This Item

실험식(Hill 표기법):
C22H12N4Na4O13S4
CAS Number:
Molecular Weight:
760.57
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47

제품명

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

Grade

for protein analysis

Quality Level

제품 라인

BioReagent

양식

liquid

농도

0.1 % (w/v) in 5% acetic acid

기술

western blot: suitable

density

1.01 g/cm3 at 20 °C

적합성

suitable (for use in cellulose acetate electrophoresis)
suitable for electrophoresis

응용 분야

diagnostic assay manufacturing
hematology
histology

저장 온도

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

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일반 설명

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

애플리케이션

Ponceau S solution has been used for membrane staining (Western blotting).

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

프로토콜

다양한 블롯 절차를 위한 워크플로 단계를 가진 웨스턴 블롯 프로토콜은 단백질이 SDS 폴리아크릴아미드 겔에서 니트로셀룰로오스 시트로 전기영동을 통해 전달되는 과정을 설명합니다. 이는 또한 면역 블로팅 프로토콜로도 알려져 있습니다.

Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.

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