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제품 라인
BioReagent
Quality Level
분석
≥90% (HPLC)
≥90% (degree of coupling )
제조업체/상표
ATTO-TEC GmbH
λ
in methanol: water (1:1) (with 0.1% perchloric acid)
UV 흡수
λ: 501-506 nm Amax
적합성
suitable for fluorescence
저장 온도
−20°C
일반 설명
Atto 488 Maleimide is a hydrophilic fluorescent label with high molecular absorption (90.000) and quantum yield (0.80). The fluorescence activity is excited at a maximum of 501nm, and the maximum emission is 523nm. Atto 488 Maleimide is optimized for argon laser excitation and characterized by high photostability. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Post coupling to a substrate, the label carries a net electrical charge of -1.
애플리케이션
Atto 488 Maleimide is used for labelling amines, which usually requires a higher pH than the reaction of maleimides with thiols. It is a well-suited fluorescent label for thiol groups.
법적 정보
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
Analysis of fluorescent nanostructures in biological systems by means of spectral position determination microscopy (SPDM).
Current Microscopy Contributions to Advances in Science and Technology, 1, 3-12 (2012)
PloS one, 7(3), e33845-e33845 (2012-04-06)
An actomyosin motor complex assembled below the parasite's plasma membrane drives erythrocyte invasion by Plasmodium falciparum merozoites. The complex is comprised of several proteins including myosin (MyoA), myosin tail domain interacting protein (MTIP) and glideosome associated proteins (GAP) 45 and
Journal of structural biology, 179(1), 68-75 (2012-05-10)
Over the last three decades, Cryo-TEM has developed into a powerful technique for high-resolution imaging of biological macromolecules in their native vitrified state. However, the method for vitrifying specimens onto EM grids is essentially unchanged - application of ∼3 μL
Thiol reactive probes and chemosensors.
Sensors, 12, 15907-15946 (2012)
Organic & biomolecular chemistry, 10(2), 273-280 (2011-11-11)
In the last few years, the use of labeled proteins has significantly expanded in the life sciences. Now, labeled proteins are indispensable tools for a wide spectrum of biophysical and chemical biology applications. In particular, the quest for more sophisticated
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