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Merck
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50980

Sigma-Aldrich

Guanidine thiocyanate

BioUltra, for molecular biology, ≥99.0% (AT)

동의어(들):

Guanidinium rhodanide, Guanidinium thiocyanate

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About This Item

Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
593-84-0
Molecular Weight:
118.16
Beilstein:
3563461
EC Number:
209-812-1
MDL number:
MFCD00013027
UNSPSC 코드:
12352107
PubChem Substance ID:
57651290
NACRES:
NA.25

Grade

for molecular biology

Quality Level

100

제품 라인

BioUltra

분석

≥99.0% (AT)

형태

solid

불순물

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

무기 잔류물

≤0.5% (as SO4)

손실

≤0.1% loss on drying, 20 °C (HV)

pH

4.5-7.0 (25 °C, 6 M in H2O)

mp

117-120 °C
120-122 °C (lit.)

solubility

H2O: 6 M at 20 °C, clear, colorless

음이온 미량물

chloride (Cl-): ≤5000 mg/kg
sulfate (SO42-): ≤50 mg/kg

양이온 미량물

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

흡수

cut-off at 280 nm in H2O at 6 M

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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관련 카테고리

애플리케이션

Chaotropic agent and strong denaturant; solubilizes cells.

기타 정보

Isolation of RNA; A new guanidine thiocyanate procedure for the isolation of full-length RNA templates for reverse transcription from tissues rich in RNase and proteoglycans

픽토그램

CorrosionExclamation mark
GHS05,GHS07

신호어

Danger

유해 및 위험 성명서

H302 + H312 + H332,H314,H412

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

보충제 위험성

EUH032

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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이미 열람한 고객

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1 of 1

Sigma-Aldrich

Sigma-Aldrich

SRE0066

Guanidine hydrochloride solution, 6M

J C Groppe et al.
Analytical biochemistry, 210(2), 337-343 (1993-05-01)
RNA isolated by conventional guanidinium isothiocyanate methods from tissues of a mollusc (red abalone: Haliotis rufescens) is largely degraded and discolored by contaminants. These contaminants are associated with inhibition of reverse transcriptase, prevent accurate spectrophotometric determination of RNA concentration, and
Turpen, T.H. and Griffith, O.M.
Biotechniques, 4, 11-11 (1986)
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is
Kai Y Mak et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(9), 1434-1443 (2015-05-23)
Angiotensin converting enzyme 2 (ACE2) which breaks down profibrotic peptide angiotensin II to antifibrotic peptide angiotensin-(1-7) is a potential therapeutic target in liver fibrosis. We therefore investigated the long-term therapeutic effect of recombinant ACE2 using a liver-specific adeno-associated viral genome
Yuji Suzuki et al.
Bioscience, biotechnology, and biochemistry, 72(7), 1951-1953 (2008-07-08)
High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus) and storage tissues (seeds
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