50981

Guanidine thiocyanate

BioReagent, for molecular biology, ≥99%

• 동의어(들): Guanidinium rhodanide, Guanidinium thiocyanate
• Linear Formula: NH₂C(=NH)NH₂ · HSCN
• CAS Number: 593-84-0
• Molecular Weight: 118.16
• Beilstein: 3563461
• EC Number: 209-812-1
• MDL number: MFCD00013027
• UNSPSC 코드: 12352107
• PubChem Substance ID: 57651291
• NACRES: NA.52

속성

• 생물학적 소스: synthetic
• Grade: for molecular biology
• 제품 라인: BioReagent
• 분석: ≥99%
• 형태: powder
• 기술: RNA extraction: suitable
• 불순물: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• 색상: colorless to white,
• mp: 115-122 °C; 120-122 °C (lit.)
• solubility: H₂O: 4 M at 20 °C, clear, colorless
• 음이온 미량물: chloride (Cl^-): ≤5000 mg/kg
• 양이온 미량물: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤5000 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• 저장 온도: room temp
• SMILES string: SC#N.NC(N)=N
• InChI: 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
• InChI key: ZJYYHGLJYGJLLN-UHFFFAOYSA-N

설명

애플리케이션

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.

Chaotropic agent and strong denaturant; solubilizes cells.

생화학적/생리학적 작용

Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.

원리

Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures.

안전성 정보

• 픽토그램: GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H302 + H312 + H332,H314,H412
• 예방조치 성명서: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• 보충제 위험성: EUH032
• Storage Class Code: 8B - Non-combustible corrosive hazardous materials
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves