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Merck
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주요 문서

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51410

Sigma-Aldrich

Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine tripotassium salt

≥98.0% (TLC)

동의어(들):

Biotin-X-NTA

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About This Item

실험식(Hill 표기법):
C26H40K3N5O9S
CAS Number:
856661-92-2
Molecular Weight:
715.98
UNSPSC 코드:
12352209
NACRES:
NA.32

Quality Level

200

분석

≥98.0% (TLC)

형태

solid

SMILES string

[K+].[K+].[K+].[O-]C(=O)CN(CC([O-])=O)[C@H](CCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)C([O-])=O

애플리케이션

Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine (Biotin-X-NTA) is used as a hetero-bifunctional linker between histone(His)-tagged proteins and (strept)avidin conjugates.
Hetero-bifunctional linker for His-tagged proteins on one side and (strept)avidin conjugates

포장

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

시험 성적서(COA)

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문서 라이브러리 방문

Eunah Kang et al.
Langmuir : the ACS journal of surfaces and colloids, 23(11), 6281-6288 (2007-04-21)
Silica surfaces modified with nitrilotriacetic acid (NTA)-polyethylene glycol (PEG) derivatives were used to immobilize hexahistidine-tagged green fluorescent protein (His6-GFP), biotin/streptavidin-AlexaFluor555 (His6-biotin/SA-AF), and gramicidin A-containing vesicles (His6-gA). Three types of surface-reactive PEG derivatives-NTA-PEG3400-Si(OMe)3, NTA-PEG3400-vinylsulfone, and mPEG5000-Si(OMe)3 (control)-were grafted onto silica and
Xiue Jiang et al.
Physical chemistry chemical physics : PCCP, 10(42), 6381-6387 (2008-10-31)
Surface enhanced infrared absorption spectroscopy (SEIRAS) has been employed to monitor the orientated assembly of a strep-tagged membrane protein on the gold surface via a streptavidin/biotin interlayer. The high surface sensitivity of SEIRAS allows for tracking the individual assembling steps
S A McMahan et al.
Analytical biochemistry, 236(1), 101-106 (1996-04-05)
Using a one-step reaction, a bifunctional compound was synthesized for detecting histidine-tagged proteins immobilized on nitrocellulose. This compound has a biotin as one functional group and a nitrilotriacetic acid as the other. The nitrilotriacetic acid is used to chelate a
Igor L Medintz et al.
Langmuir : the ACS journal of surfaces and colloids, 21(12), 5501-5510 (2005-06-01)
This report describes two related methods for decorating cowpea mosaic virus (CPMV) with luminescent semiconductor nanocrystals (quantum dots, QDs). Variants of CPMV are immobilized on a substrate functionalized with NeutrAvidin using modifications of biotin-avidin binding chemistry in combination with metal
D J O'Shannessy et al.
Analytical biochemistry, 229(1), 119-124 (1995-07-20)
The use of short peptide affinity tag sequences has become commonplace for the expression and purification of recombinant proteins. Many of these tags are antibody epitopes and detection of tagged proteins via Western blots is straightforward. However, the most common
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