71183

Sodium acetate

anhydrous, BioUltra, for luminescence, for molecular biology, ≥99.0% (NT)

• 동의어(들): Sodium acetate anhydrous, Acetic acid Sodium salt anhydrous, Acetic acid sodium salt
• Linear Formula: CH₃COONa
• CAS Number: 127-09-3
• Molecular Weight: 82.03
• Beilstein: 3595639
• EC Number: 204-823-8
• MDL number: MFCD00012459
• UNSPSC 코드: 12161700
• eCl@ss: 39021906
• PubChem Substance ID: 57652226
• NACRES: NA.25

속성

• Grade: for luminescence; for molecular biology
• Quality Level: 200
• 제품 라인: BioUltra
• 분석: ≥99.0% (NT)
• 형태: solid
• autoignition temp.: 1112 °F
• 불순물: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• 손실: ≤0.5% loss on drying, 150 °C
• pH: 8.5-10.0 (25 °C, 3 M in H₂O)
• pKa: 4.76 (acetic acid)
• mp: >300 °C (dec.) (lit.)
• solubility: H₂O: 3 M at 20 °C, clear, colorless
• 음이온 미량물: chloride (Cl^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• 양이온 미량물: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤10 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤200 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤10 mg/kg; Zn: ≤5 mg/kg
• SMILES string: [Na+].CC([O-])=O
• λ: 3 M in H₂O
• UV 흡수: λ: 260 nm A_max: 0.03; λ: 280 nm A_max: 0.02
• InChI: 1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1
• InChI key: VMHLLURERBWHNL-UHFFFAOYSA-M

설명

애플리케이션

Sodium acetate is suitable for use as a buffer in:

• protein crystallization
• staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography
• the preparation of standard (acetylcholine chloride) for hestrin assay
• substrate screening characterization for microfluidic microbial fuel cell array

생화학적/생리학적 작용

Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather.

기타 정보

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)