A0856

Anti-ATG5 (N-terminal) antibody produced in rabbit

affinity isolated antibody, PBS solution

• 동의어(들): ATG5 Antibody - Anti-ATG5 (N-terminal) antibody produced in rabbit, Atg5 Antibody, Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog (S. cerevisiae), Anti-Apoptosis-specific protein, Anti-Autophagy protein 5-like
• MDL number: MFCD09753847
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: PBS solution
• 분자량: antigen ~56 kDa (Atg5-Atg12 complex)
• 종 반응성: mouse, human, rat
• 포장: antibody small pack of 25 μL
• 기술: immunofluorescence: suitable; western blot: 0.5-1 μg/mL using whole extracts of human K562, rat NRK, and mouse 3T3 cells
• UniProt 수납 번호: Q9H1Y0
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ATG5(9474) ; mouse ... Atg5(11793) ; rat ... Atg5(365601)

설명

특이성

Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.

면역원

synthetic peptide corresponding to amino acids 2-15 of human ATG5, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

애플리케이션

Anti-ATG5 (N-terminal) antibody has been used:

• in immunoblotting
• in immunofluorescence
• in western blotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5.

Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.

생화학적/생리학적 작용

Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)