L8918

Anti-MAP1LC3A antibody

rabbit polyclonal

• 동의어(들): Anti-Lc3, LC3 Antibody - Anti-LC3 antibody produced in rabbit, Lc3 Antibody, Anti-MAP1 light chain 3-like protein 1, Anti-MAP1A/1B light chain 3A, Anti-MAP1A/1BLC3, Anti-MAP1A/MAP1B LC3A, Anti-MAP1ALC3, Anti-MAP1BLC3, Anti-MAP1LC3A, Anti-MAP1LC3B, Anti-Microtubule-associated protein 1 light chain 3 alpha, Anti-Microtubule-associated protein 1 light chain 3 beta, Anti-Microtubule-associated proteins 1A/1B light chain 3
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 제품명: Anti-LC3 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen 16-18 kDa
• 종 반응성: mouse, human, rat
• 포장: antibody small pack of 25 μL
• 농도: ~1 mg/mL
• 기술: immunohistochemistry: suitable; immunoprecipitation (IP): 5-10 μg using extracts of human U87 cells; western blot: 1-2 μg/mL using whole extracts of rat and mouse brain
• UniProt 수납 번호: Q9H492
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MAP1LC3A(84557) , MAP1LC3B(81631) ; mouse ... Map1lc3a(66734) , Map1lc3b(67443) ; rat ... Map1lc3a(362245) , Map1lc3b(64862)

설명

일반 설명

The gene LC3, also referred to as MAP1LC3A (microtubule associated protein 1 light chain 3 α), is a homologue of Apg8p necessary for autophagy in yeast. The cytosolic form upon processing gets localized to the autophagosome membranes. The gene is mapped to human chromosome 20q11.22. LC3 exists in cells as cytoplasmic LC3-I (18kDa) and LC3-II (16kDa).

면역원

Synthetic peptide corresponding to amino acids of human LC3A isoform a, conjugated to KLH via C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

애플리케이션

Anti-LC3 antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence
• immunohistochemistry
• filter retardation assay
• immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (3 papers)
Immunohistochemistry (1 paper)
Western Blotting (21 paper)

생화학적/생리학적 작용

The gene MAP1LC3A (microtubule associated protein 1 light chain 3 α) encodes a protein that serves as a marker of autophagosomes. It is found to be expressed on surviving oligodendrocytes in Nasu-Hakola disease brains and may be involved in the induction of oligodendrogliopathy.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)