A1287

5-Azacytidine

Hybri-Max^™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

• 동의어(들): 4-Amino-1-(β-D-ribofuranosyl)-1,3,5-triazin-2(1H)-one, Ladakamycin
• 실험식(Hill 표기법): C₈H₁₂N₄O₅
• CAS Number: 320-67-2
• Molecular Weight: 244.20
• Beilstein: 620461
• EC Number: 206-280-2
• MDL number: MFCD00006539
• UNSPSC 코드: 12352207
• PubChem Substance ID: 24890525
• NACRES: NA.75

속성

• 생물학적 소스: synthetic (organic)
• Quality Level: 200
• Grade: Hybri-Max^™
• 무균: γ-irradiated
• 제품 라인: BioXtra
• 양식: lyophilized powder
• 기술: cell culture | hybridoma: suitable
• 불순물: endotoxin, tested
• mp: 226-232 °C (dec.) (lit.)
• solubility: acetic acid: water (1:1): 5 mg/mL, clear, colorless
• 항생제 활성 스펙트럼: viruses
• 동작 모드: DNA synthesis | interferes
• 저장 온도: −20°C
• SMILES string: NC1=NC(=O)N(C=N1)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O
• InChI: 1S/C8H12N4O5/c9-7-10-2-12(8(16)11-7)6-5(15)4(14)3(1-13)17-6/h2-6,13-15H,1H2,(H2,9,11,16)/t3-,4-,5-,6-/m1/s1
• InChI key: NMUSYJAQQFHJEW-KVTDHHQDSA-N
• 유전자 정보: human ... DNMT1(1786) , DNMT3A(1788)

설명

일반 설명

Chemical structure: nucleoside

애플리케이션

5-Azacytidine may be used to induce in vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. It may also be used to study the effects of 5-Azacytidine on the immune regulatory myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs).

Used to study the effects of the DNA incorporated molecule on DNA methylation processes.

생화학적/생리학적 작용

5-Azacytidine is a deoxycytidine analog and a demethylating agent. It acts as a potential antineoplastic agent for acute myelogenous leukemia. 5-Azacytidine activates repressed genes by inhibiting DNA methylation. 5-Azacytidine also affects protein synthesis by altering the RNA function and stability.

A potent growth inhibitor and cytotoxic agent; inhibits DNA methyltransferase, an important regulatory mechanism of gene expression, gene activation and silencing.

재구성

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500ml medium. Final working concentration: 10 μM 5-azacytidine.

법적 정보

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• 픽토그램: GHS08,GHS07,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302,H341,H350,H360FD,H372,H410
• 예방조치 성명서: P202 - P260 - P264 - P273 - P301 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1B - STOT RE 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges