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Merck
모든 사진(1)

주요 문서

A1559

Sigma-Aldrich

Monoclonal Anti-Biotin–Agarose antibody produced in mouse

clone BN-34, purified immunoglobulin, PBS suspension

동의어(들):

Monoclonal Anti-Biotin

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

mouse

Quality Level

재조합

expressed in mouse cell line

결합

agarose conjugate

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

BN-34, monoclonal

양식

PBS suspension

라벨링 범위

2 mg antibody per mL resin

기술

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG1

용량

0.15-0.3 μmol/mL, agarose binding capacity (biotin-ligand conjugate)

응용 분야

research pathology

배송 상태

wet ice

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

면역원

Biotin-KLH conjugate

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
May be substituted for avidin to decrease non-specific binding.
Monoclonal Anti-Biotin-Agarose antibody produced in mouse has been used in:
  • pulldown assay
  • coimmunoprecipitation 
  • chromatin immunoprecipitation
  • purification of biotinylated compounds and biotin-containing compounds
Mouse monoclonal anti-biotin antibody was used to identify by western blot S-nitrosylated proteins that had been labeled with a biotin moeity based on the biotin-switch method. No reactivity was visible on the blots when the biotin switch method was inhibited by treatment with 100 mM dithiothreitol showing that this antibody shows no unspecific cross reactivity. The mouse monoclonal anti-biotin antibody does cross react with Arabidopsis plant by western blot, no cross reactivity with unbiotinylated proteins.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

물리적 형태

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

제조 메모

Prepared using purified antibody coupled to cyanogen bromide activated agarose

기타 정보

Polyclonal anti-biotin antibodies were shown to react with in vivo biotinylated proteins but not monoclonal anti-biotin antibodies.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

A two-step strategy to visually identify molecularly imprinted polymers for tagged proteins
Brandis A, et al.
Journal of Separation Science, 40(16), 3358-3367 (2017)
Namrata D Udeshi et al.
Nature methods, 14(12), 1167-1170 (2017-10-19)
Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide mixtures. Live-cell proximity labeling using APEX peroxidase followed by anti-biotin enrichment
Transient dsDNA breaks during pre-replication complex assembly
Rampakakis E and Zannis-Hadjopoulos M
Nucleic Acids Research, 37(17), 5714-5724 (2009)
Protein phosphatase 1c associated with the cardiac sodium calcium exchanger 1 regulates its activity by dephosphorylating serine 68-phosphorylated phospholemman
Hafver T, et al.
The Journal of biological chemistry, 291(9), 4561-4579 (2016)
Alexander Brandis et al.
Journal of separation science, 40(16), 3358-3367 (2017-06-13)
A practical and relatively simple method to identify molecularly imprinted polymers capable of binding proteins via the molecular tagging (epitope-like) approach has been developed. In our two-step method, we first challenge a previously obtained anti-tag molecularly imprinted polymer with a

관련 콘텐츠

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

친화성, GST 풀다운, TAP, 공동 면역 침전법을 활용한 풀다운 분석을 통해 in vitro 단백질-단백질 상호작용을 분석합니다.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.