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Merck
모든 사진(6)

문서

A2383

Sigma-Aldrich

Adenosine 5′-triphosphate disodium salt hydrate

Grade I, ≥99%, from microbial

동의어(들):

ATP disodium salt

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About This Item

실험식(Hill 표기법):
C10H14N5Na2O13P3 · xH2O
CAS Number:
Molecular Weight:
551.14 (anhydrous basis)
EC Number:
MDL number:
UNSPSC 코드:
41106305
eCl@ss:
32160414
PubChem Substance ID:
NACRES:
NA.51

생물학적 소스

microbial

Quality Level

유형

Grade I

분석

≥99%

형태

powder

solubility

H2O: 50 mg/mL

저장 온도

−20°C

SMILES string

O.[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O

InChI

1S/C10H16N5O13P3.2Na.H2O/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;;1H2/q;2*+1;/p-2/t4-,6-,7-,10-;;;/m1.../s1

InChI key

NTBQNWBHIXNPRU-MSQVLRTGSA-L

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애플리케이션

Adenosine 5′-triphosphate disodium salt hydrate has been used:
  • to prepare adenosine triphosphate (ATP) standard solutions to determine ATP levels in various bacterial cultures
  • bone marrow-derived macrophages (BMDM) treatment
  • as a preparation free of contaminating guanine nucleotides to study the binding effects of coatomer β subunit (β-COP)

생화학적/생리학적 작용

Adenosine 5′-triphosphate (ATP) is a central component of energy storage and metabolism in vivo. ATP is used in many cellular processes, respiration, biosynthetic reactions, active transport, and motility. ATP is a substrate of many kinases involved in cell signaling and of adenylate cyclase that produce the secondary messenger cyclic adenosine monophosphate (cAMP). ATP provides the metabolic energy to drive metabolic pumps. It serves as a coenzyme in a wide array of enzymatic reactions.
P2 purinergic agonist; increases activity of Ca2+-activated K+ channels; substrate for ATP-dependent enzyme systems

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Faceshields, Gloves, type N95 (US)


시험 성적서(COA)

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문서 라이브러리 방문

Release of extracellular ATP by bacteria during growth
Mempin R, et al.
BMC Microbiology, 13(1), 301-301 (2013)
J G Donaldson et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(14), 6408-6412 (1992-07-15)
The coatomer is a cytosolic protein complex that reversibly associates with Golgi membranes and is implicated in modulating Golgi membrane transport. The association of beta-COP, a component of coatomer, with Golgi membranes is enhanced by guanosine 5'-[gamma-thio]triphosphate (GTP[gamma S]), a
Rui Nian et al.
Biotechnology progress, 25(4), 1078-1085 (2009-06-25)
The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperone-mediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the
Mass spectrometry and NMR spectroscopy based quantitative metabolomics
Proteomics. Clinical Applications (2020)
Physiology, Adenosine Triphosphate (ATP)
NCBI Bookshelf null

프로토콜

Measure luciferase activity using a luminometer assay detecting light emission, with applications in ATP detection and genetic function reporting.

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

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