A8111

Plasminogen activator inhibitor 1 (PAI-1)

≥200,000 units/mg protein, lyophilized powder, ≥90% (SDS-PAGE)

• 동의어(들): PAIE, PLANH1, Plasminogen activator inhibitor, β-migrating endothelial cell-derived type, Serpin E1 PAI-1, Serpine 1
• CAS Number: 140208-23-7
• MDL number: MFCD01094930
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• product name: Plasminogen activator inhibitor 1 (PAI-1) human, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
• 재조합: expressed in E. coli
• Quality Level: 200
• 분석: ≥90% (SDS-PAGE)
• 형태: lyophilized powder
• 특이 활성도: ≥200,000 units/mg protein
• 분자량: ~43 kDa
• solubility: water: 0.1 mL, clear, colorless
• UniProt 수납 번호: P05121
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... SERPINE1(5054)

설명

일반 설명

Plasminogen activator inhibitor 1 (PAI-1) or serine protease inhibitor E1 (SERPINE1) gene is mapped to human chromosome 7q22.1. PAI-1 is produced in hepatocytes, endothelial cells, smooth muscle cells, megakaryocytes, and adipocytes.

애플리케이션

Plasminogen activator inhibitor 1 (PAI-1) human has been used:

• as an inhibitor of pro-brain-derived neurotrophic factor (proBDNF) in radioimmunoprecipitation assay (RIPA) in peripheral blood and lymphocytes
• to block plasmin activation in mice infected with C. albicans hyphae
• to test its effect on neurite growth in neurons

생화학적/생리학적 작용

Plasminogen Activator Inhibitor 1 (PAI-1) protein belongs to the Serpin superfamily of serine protease inhibitors. It is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and therefore inhibits the fibrinolysis process. PAI is involved in extracellular matrix remodeling and has been implicated in processes like angiogenesis, chemotaxis, ovulation, and embryogenesis. PAI-1 is present in increased levels in various disease states such as a number of cancer forms, obesity and the metabolic syndrome.

Plasminogen activator inhibitor–1 (PAI -1) participates in mediating cellular senescence. It is upregulated during injury along with other tissue repair transcriptomes. Elevated levels of PAI-1 is regarded as one of the risks associated with myocardial infarction (MI).

단위 정의

1 unit irreversibly inhibits (100% inhibition) 1 unit of tissue plasminogen activator (tPA) under physiological conditions (neutral pH and 37 ºC).

물리적 형태

Supplied as a lyophilized powder from a solution containing 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.

재구성

Reconstitute to 0.25 mg/ml with ultrapure water. The reconstituted product contains 0.25 mgP/mL in 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.

분석 메모

The product is the active human PAI-1 protein with a molecular weight of 43 kDa.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable