A8481

Anti-γ-Actin antibody, Mouse monoclonal

clone 2-2.1.14.17, purified from hybridoma cell culture

• 동의어(들): Anti-ACTG1
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: 2-2.1.14.17, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~43 kDa
• 종 반응성: mouse, chicken, canine, bovine, human, hamster
• 포장: antibody small pack of 25 μL
• 농도: ~2 mg/mL
• 기술: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of 3T3 cells
• 동형: IgG1
• UniProt 수납 번호: P63261
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: bovine ... ACTG1(404122) ; chicken ... ACTG1(415296) ; human ... ACTG1(71) ; mouse ... Actg1(11465)

설명

일반 설명

γ-Actin is mainly present in sarcolemma and to a lesser extent in reticular lattice of normal muscles. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.

Monoclonal Anti-γ-Actin (mouse IgG1 isotype) is derived from the hybridoma 2-2.1.14.17 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Actin isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 NH₂-terminal residues. Actin constitutes for around 15% of the total protein. It exists in globular and filamentous forms.

특이성

Monoclonal Anti-γ-Actin recognizes human, bovine, canine, mouse, hamster and chicken γ-actin.

애플리케이션

Anti-γ-Actin antibody, Mouse monoclonal has been used in :

• immunofluorescence
• enzyme linked immunosorbent assay (ELISA)
• western blotting
• immunohistochemistry

Mouse monoclonal Anti-γ-Actin antibody has been used for immunofluorescence assays. The antibody can also be used for ELISA, IHC and western blot assays.

Protein lysates from human Jurkat T-leukemic cells were subjected to western blot and probed using monoclonal mouse anti-actin (AC-40) to ensure equal loading.

생화학적/생리학적 작용

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin is significantly involved in the microfilament system. It participates in muscle contraction and gene regulation. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

기타 정보

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)