추천 제품
애플리케이션
A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
BCIP®/NBT Liquid Substrate System has been used:
- for staining alkaline phosphatase(ALP) in bone marrow stromal cells
- as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
- as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays
주의사항
It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.
법적 정보
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
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문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
프로토콜
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
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