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Merck
모든 사진(7)

주요 문서

B6755

Sigma-Aldrich

BIS-TRIS propane

≥99.0% (titration)

동의어(들):

1,3-Bis[tris(hydroxymethyl)methylamino]propane, 2,2′-(Propane-1,3-diylbis(azanediyl))bis(2-(hydroxymethyl)propane-1,3-diol)

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About This Item

Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
Molecular Weight:
282.33
Beilstein:
1786109
EC Number:
MDL number:
UNSPSC 코드:
12161700
PubChem Substance ID:
NACRES:
NA.25

Quality Level

분석

≥99.0% (titration)

양식

powder

저장 조건

dry at room temperature

기술

HPLC: suitable

색상

white

유용한 pH 범위

6.3-9.5

pKa(25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

solubility

water: 30 % (w/w), clear, colorless

density

1.179 g/cm3 at 20—25 °C
1.189 g/cm3 at 20—25 °C

적합성

suitable for chromatography
suitable for electrophoresis buffers

응용 분야

diagnostic assay manufacturing
general analytical
life science and biopharma

저장 온도

room temp

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

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일반 설명

Bis-Tris Propane buffer, also known as BTP, is a zwitterionic biochemical buffer utilized for pH regulation in various laboratory applications, particularly in molecular biology, biochemistry, and electrophoresis. Its wide buffering range, spanning from pH 6.3 to 9.5, is attributed to its two pKa values of 6.8 (pKa1) and 9.0 (pKa2), which are close. This wide range makes it highly versatile for maintaining stable pH conditions in a variety of experiments.

Furthermore, Bis-Tris Propane is a water-soluble buffer substance that plays a crucial role in enhancing the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments that require pH levels as low as 6-7. When compared to Tris buffer, which has a poor buffering capacity below pH 7.5 and exhibits significant pKa fluctuations with temperature changes, Bis-Tris Propane proves to be a superior choice. Additionally, it finds application in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly when isolated from Saccharomyces cerevisiae strains. Notably, it can also serve as a ligand, forming dinuclear hydroxo complexes with Lanthanides(III).

애플리케이션

BIS-TRIS Propane may be used in the following studies:
  • as a buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO2).
  • for purification of glucose-binding protein from membranes of Sulfolobus solfataricus.
  • as a buffer during GeO2 mineralisation.
  • as a buffer component in a study pertaining to environmental research
  • as a buffer component to maintain optimal pH conditions during the purification and preparation of engineered enzymes

특징 및 장점

  • Suitable for Biological and Biochemical Research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Effective Buffering from pH 6.3-9.5 (25 °C) with a pKa of 6.8 and 9.0 (25 °C)
  • Highly soluble in water

기타 정보

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

이미 열람한 고객

Engineering the Organophosphorus Acid Anhydrolase Enzyme for Increased Catalytic Efficiency and Broadened Stereospecificity on Russian VX
Daczkowski CM et al.
Biochemistry, 54, 6423-6433 (2015)
Y Satoh et al.
The Journal of biological chemistry, 256(20), 10272-10275 (1981-10-25)
In the presence of a salt in an excess amount, the oxidation of native MbO2 was enhanced considerably above the normal autoxidation in buffr alone with the formation of the corresponding MetMb . anion complex. This anion-induced oxidation of MbO2
S V Albers et al.
Journal of bacteriology, 181(14), 4285-4291 (1999-07-10)
The archaeon Sulfolobus solfataricus grows optimally at 80 degrees C and pH 2.5 to 3.5 on carbon sources such as yeast extracts, tryptone, and various sugars. Cells rapidly accumulate glucose. This transport activity involves a membrane-bound glucose-binding protein that interacts
Elisabeth Dohmann Chang et al.
Environmental science & technology, 55(10), 6848-6856 (2021-03-17)
Water pH is predicted to affect the uptake of ionizable pharmaceuticals in fish. The current study used an in vitro primary fish gill cell culture system to assess the effect of pH values in the range of 4.5-8.75 on the
Estefania Boix et al.
Dalton transactions (Cambridge, England : 2003), 43(44), 16902-16910 (2014-10-11)
We demonstrate the use of silk based proteins to control the particle/crystallite size during GeO2 formation, using a bio-mimetic approach at circumneutral pH and ambient temperature. Multicrystalline GeO2 was prepared from germanium tetraethoxide (TEOG) in the presence of different silk-based

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