B9754

BIS-TRIS

≥98.0% (titration)

• 동의어(들): 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
• 실험식(Hill 표기법): C₈H₁₉NO₅
• CAS Number: 6976-37-0
• Molecular Weight: 209.24
• Beilstein: 2205275
• EC Number: 230-237-7
• MDL number: MFCD00002853
• UNSPSC 코드: 12161700
• PubChem Substance ID: 24892155
• NACRES: NA.25

속성

• Quality Level: 400
• 분석: ≥98.0% (titration)
• 양식: crystalline powder
• 저장 조건: dry at room temperature
• 기술: UV/Vis spectroscopy: suitable; electrophoresis: suitable
• 색상: white
• 유용한 pH 범위: 5.8-7.2
• pKa(25 °C): 6.5
• solubility: H₂O: 500 mg/mL, clear, colorless to very faintly yellow
• 적합성: suitable for Western blot; suitable for column chromatography of proteins
• 응용 분야: diagnostic assay manufacturing; general analytical; microbiology
• SMILES string: OCCN(CCO)C(CO)(CO)CO
• InChI: 1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
• InChI key: OWMVSZAMULFTJU-UHFFFAOYSA-N

설명

일반 설명

Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.

애플리케이션

Bis-Tris has been used :

• as a buffer for Electrophoresis
• as a component of transfer buffer for Western Blotting

특징 및 장점

• Suitable for Biological and Biochemical Research
• Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C)

기타 정보

For additional information on our range of Biochemicals, please complete this form.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H319
• 예방조치 성명서: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves