B7651

Brefeldin A

from Penicillium brefeldianum, ≥99% (HPLC and TLC), powder, activator of the sphingomyelin cycle

• 동의어(들): Nectrolide, γ,4-Dihydroxy-2-(6-hydroxy-1-heptenyl)-4-cyclopentanecrotonic acid λ-lactone, Ascotoxin, BFA, Cyanein, Decumbin
• 실험식(Hill 표기법): C₁₆H₂₄O₄
• CAS Number: 20350-15-6
• Molecular Weight: 280.36
• Beilstein: 25191
• MDL number: MFCD12913297
• UNSPSC 코드: 12352200
• PubChem Substance ID: 24278285
• NACRES: NA.77

속성

• product name: Brefeldin A, from Penicillium brefeldianum, ≥99% (HPLC and TLC)
• 생물학적 소스: Penicillium brefeldianum
• Quality Level: 300
• 분석: ≥99% (HPLC and TLC)
• 형태: powder
• solubility: DMSO: 10 mg/mL
• 항생제 활성 스펙트럼: neoplastics
• 동작 모드: protein synthesis | interferes
• 저장 온도: 2-8°C
• SMILES string: C[C@H]1CCC\C=C\[C@@H]2C[C@H](O)C[C@H]2[C@H](O)\C=C\C(=O)O1
• InChI: 1S/C16H24O4/c1-11-5-3-2-4-6-12-9-13(17)10-14(12)15(18)7-8-16(19)20-11/h4,6-8,11-15,17-18H,2-3,5,9-10H2,1H3/b6-4+,8-7+/t11-,12+,13-,14+,15+/m0/s1
• InChI key: KQNZDYYTLMIZCT-KQPMLPITSA-N

설명

일반 설명

Brefeldin A (BFA) is a fungal metabolite containing a 13-membered macrocyclic lactone ring. It inhibits protein and nucleic acid synthesis. BFA blocks the exchange of GDP for GTP on adenosine ribosylation factors (ARFs), thereby hindering the binding of coat protein (β-COP) and consequently disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells. Brefeldin A has been shown to increase CRISPR-mediated homology-directed repair (HDR) efficiency. It demonstrates a diverse array of biological functions, including antitumor, antiviral, antibiotic, antimitotic, antifungal and antinematodal properties.

애플리케이션

Brefeldin A has been used to:

• increase CRISPR genome editing efficiency.
• facilitate the measurement of cytokine production
• block intracellular transports in cell culture experiments

생화학적/생리학적 작용

Brefeldin A (BFA) is a fungal metabolite which disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells.

Brefeldin A has been shown to increase CRISPR-mediated homology-directed repair (HDR) efficiency.

안전성 정보

• 픽토그램: GHS06
• 신호어: Danger
• 유해 및 위험 성명서: H301
• 예방조치 성명서: P301 + P310
• Hazard Classifications: Acute Tox. 3 Oral
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves