추천 제품
재조합
expressed in E. coli (C-terminal histidine-tagged)
Quality Level
분석
≥90% (SDS-PAGE)
형태
buffered aqueous glycerol solution
특이 활성도
≥1.0 units/mg protein
분자량
~30 kDa
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−70°C
유전자 정보
human ... CASP3(836)
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일반 설명
Research area: Apoptosis
Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag.
Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag.
애플리케이션
Caspase 3 human has been used to digest human r-vimentin. It has also been used in caspase-3 inhibition assay and protease inhibition assay.
This product may also be used in cell-based apoptosis assay.
This product may also be used in cell-based apoptosis assay.
생화학적/생리학적 작용
Caspase 3 is a member of the CED-3 subfamily of caspases and is responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation. Caspase 3 is considered to be an effector caspase, activating pro-caspase 6 and pro-caspase 9 in vitro. Caspase 3 can be activated by caspase 8, caspase 6, and granzyme B.
단위 정의
One unit will cleave 1.0 μmole of N-acetyl-Asp-Glu-Val-Asp-pNA per min at pH 7.4 at 25 °C.
물리적 형태
Solution in 10% (w/v) glycerol containing 50 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS
제조 메모
Caspase 3 is synthesized as a 32 kDa proenzyme. The active enzyme is a heterodimer of two large (17 kDa) subunits and two small (12 kDa) subunits.
기질
제품 번호
설명
가격
억제제
항체
제품 번호
설명
가격
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
A peptide-based positron emission tomography probe for in vivo detection of caspase activity in apoptotic cells
Hight MR, et al.
Clinical Cancer Research, 20(8), 2126-2135 (2014)
Cross-presentation of caspase-cleaved apoptotic self antigens in HIV infection
Rawson PM, et al.
Nature Medicine, 13(12), 1431-1431 (2007)
Deju Ye et al.
Nature chemistry, 6(6), 519-526 (2014-05-23)
Directed self-assembly of small molecules in living systems could enable a myriad of applications in biology and medicine, and already this has been used widely to synthesize supramolecules and nano/microstructures in solution and in living cells. However, controlling the self-assembly
Regulation of spermatogenic cell apoptosis by the pro-apoptotic proteins in the testicular tissues of mammalian and avian species
Zakariah M, et al.
Animal Reproduction Science (2022)
Petra Procházková et al.
PloS one, 9(10), e109900-e109900 (2014-10-04)
Iron homeostasis in cells is regulated by iron regulatory proteins (IRPs) that exist in different organisms. IRPs are cytosolic proteins that bind to iron-responsive elements (IREs) of the 5'- or 3'-untranslated regions (UTR) of mRNAs that encode many proteins involved
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