C2037

Cytochrome c from bovine heart

≥95% based on Mol. Wt. 12,327 basis

• CAS Number: 9007-43-6
• EC Number: 232-700-9
• MDL number: MFCD00130890
• UNSPSC 코드: 12352202
• NACRES: NA.61

속성

• 생물학적 소스: bovine heart
• Quality Level: 300
• 분석: ≥95% based on Mol. Wt. 12,327 basis
• 형태: powder or crystals
• 분자량: 12327 Da
• 기술: cell based assay: suitable
• 적합성: suitable for molecular biology
• UniProt 수납 번호: P00125
• 응용 분야: cell analysis
• 저장 온도: −20°C
• 유전자 정보: cow ... CYC1(512500)

설명

일반 설명

Cytochrome C is a heme protein. It is localized between the inner and outer mitochondrial membranes.

애플리케이션

Cytochrome c from bovine heart has been used:

• for cytochrome oxidase staining using the mice brain tissue
• as a component of control mixture to evaluate the accuracy of tandem mass spectra in identifying peptides
• to study its effect on the stability, transport, and retention of biochar colloids (BCs) in saturated porous media under various solution pH and ionic strength (IS) conditions
• as a component of staining solution for the detection of cytochrome oxidase activity in the brain tissue sections of mouse
• as a component in the assay to study its superoxide scavenging efficiency
• as a component of serum-free DMEM media to evaluate non-specific cytochrome C reduction in astrocytes and HT-29 cells

생화학적/생리학적 작용

Cytochrome C is a key mediator of oxidative phosphorylation. It mediates the electron transport between complex III and complex IV of the respiratory chain.

Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are an inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome.

The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.

제조 메모

Prepared using TCA.

기타 정보

View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)