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Merck
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C5467

SAFC

EX-CELL® ACF CHO Medium

Animal-component free, with HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

의약품 제조

동의어(들):

CHO Medium

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About This Item

MDL number:
MFCD01634638
UNSPSC 코드:
12352207
NACRES:
NA.75

Quality Level

500

설명

for research or for further manufacturing use

무균

sterile-filtered

양식

liquid

품질

Drug/Device Master File available

기술

cell culture | mammalian: suitable

성분

HEPES: yes
NaHCO3: yes
phenol red: no
L-glutamine: no

배송 상태

ambient

저장 온도

2-8°C

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관련 카테고리

애플리케이션

Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.

특징 및 장점

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity. Cell densities in excess of 8 × 106 cells/ml have been achieved in batch culture systems. Increases in protein productivity up to 500% per cell have been observed.

기타 정보

Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.

재구성

Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.

법적 정보

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number
0000288080
RNBL8517
RNBL3238
RNBK8042
SLCP5281

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문서

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

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