A431 Cell Line human

85090402, human skin, Epithelial

• 동의어(들): A-431 Cells, A431/P Cells
• UNSPSC 코드: 41106514

속성

• 제품명: A431 Cell Line human, squamous carcinoma, 85090402
• 생물학적 소스: human skin
• 성장 모드: Adherent
• 핵형: Hypertriploid
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Epidermal growth factor (EGF)
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human squamous carcinoma

세포주 설명

Derived from an epidermal carcinoma of the vulva taken from an 85 yr old female. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.

애플리케이션

A431 Cell Line human has been used:

• to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
• to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
• to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants

Cell cycle studies

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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