HT29 Cell Line human

91072201, human colon, Epithelial

• 동의어(들): HT29 Cells
• UNSPSC 코드: 41106514

속성

• product name: HT29 Cell Line human, 91072201, Caucasian colon adenocarcinoma grade II
• 생물학적 소스: human colon
• 성장 모드: Adherent
• 핵형: 2n = 46, hypertriploid
• 형태학: Epithelial
• 제품: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian colon adenocarcinoma

세포주 설명

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

애플리케이션

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.

Tumourigenicity studies

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

배양 배지

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

기타 정보

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